ABSTRACT
The Ehretiaabyssinica, a small tree, belong to Boraginaceae family is traditionally known for its medicinal properties. The present study was therefore carried out to investigate the antibacterial activities and to unveil the Phytochemicals of the bioactive components in the leaves extract of this plant species. The antimicrobial activities of leaves extract was investigated against 7 medically important bacterial strains, namely Bacillus subtilius, MRSA, Micrococcus ,Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aurues and Klebsella pneumoniae. The antibacterial activity was determined using agar well diffusion method. The most susceptible bacteria to this extract were Pseudomonas aeruginosa, followed by Staphylococcus aurues, while the most resistant bacteria were Micrococcus. GC-MS analysis revealed that the ethanol leave extract of Ehretiaabyssinica contained mainly Octadecenamide (5.77%); Lucenin 2 (5.46%); Docosane and Nonacosane (3.75%); Cyclopropene (3.50%); Hematoporphyrin (2.68%); Tetratetracontane (2.36%); Dotriacontane (1.57); Acetic acid (1.53); Nmethylglycine (1.49%); Propyne antimicrobial (1.41%). All identified compounds are known to have antimicrobial activity.
ABSTRACT
Conocarpus erectus L. (Family Combretaceae) is an introduced plant species, widely spread in Jeddah City, and other parts of Saudi Arabia. Folk medicine has used Parts of this species in its native countries. In the present work four different concentrations of ethanol extracts (50; 100; 150 & 200ppm) from different parts of this plant (seeds, stem, leaves and bark) were assessed against three Gram+ve bacteria (Bacillus subtilius, Micrococcus luteus, Staphylococcus aureus) and three Gram-ve bacteria (Escherichia coli, Pseudomonas aeruginosa & Klebsiella pneumoniae).The effect magnitude of these extracts on tested bacteria strains was as follows: leaves extracts bark stem seeds. Nevertheless results showed a strong activity of all thesedifferent parts against Staphylococcus aureus among the Gram+ve bacteria. The maximum inhibitory effect was observed at 200 μL /dish by all used parts extracts except the bark extract against Pseudomonas aeruginosa, followed by Escherichia coli and Klebsiella pneumoniae among the Gram–Ve bacteria. The Random Amplified Polymorphic DNA (RAPD) assay was used to assess the level of DNA damage in treated bacteria strains. The results confirmed the potentials of Conocarpus erectus as good antibacterial source. Worth noting that the present report, is the first on the antibacterial and molecular characterization of bacteria strains by Conocarpus erectus, growing in the west coast of Saudi Arabia. In conclusion we recommend more innovative investigations, in order to explore the treasures of this therapeutically important plant species.